My projects
Photo Video Blog Books
Goltsov Sergey
I serve the art of medical doubt

METHOD FOR DETERMINING SUBPOPULATION COMPOSITION OF SKIN CELLS AND OBTAINING SKIN CYTOIMMUNOGRAM

FIELD: medicine.

SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.

EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.

 

Ещё в блоге:

Khanty, Nenets and… Masai!
Khanty, Nenets and… Masai!

Казалось бы, что может быть общего между коренными народами Крайнего Севера и африканцами? При всем желании очень трудно найти сходство между ненцами, хантами и представителями племен датогов и масаи, проживающих в Танзании. С таким же успехом можно пытаться сравнить раскаленный африканский песок и жидкий азот, лед и пламя, жару и холод, черное и белое. Тем не менее, побывав во время недавней экспедиции в Африке, удалось увидеть то, что роднит народы, никогда не пересекавшиеся друг с другом.

Lecture "Diagnosis: The Traveler"

International Festival in Travel and Tourism named after N.N. Miklouho-Maclay "Russian traveler" was held in Orel.

My lecture "Diagnosis: The Traveler", planned in three parts, one for each day of the festival, was received with interest by world-famous travelers. But, as they say, tell God about your plans and he will laugh - and it happened. The third part, in my opinion the most important, because of the time-bound regulation was not brought to the listeners. However, in this there was a plus, as this is the best reason to continue the relationship outside the festival and breaking the boundaries, regardless of the distance to complete the scheduled. Let's do it, my friends!

CERTAIN IMMUNOPFYSIOLOGICAL MECHANISMS OF WOUND REPAIR IN CONDITION OF WOUND HEALING MADICAL MEANS «Cellgel» APPLICATION
CERTAIN IMMUNOPFYSIOLOGICAL MECHANISMS OF WOUND REPAIR IN CONDITION OF WOUND HEALING MADICAL MEANS «Cellgel» APPLICATION

Проведен эксперимент на 40 половозрелых кроликах с целью сравнения пролиферативной и апоптотической активности клеток эпидермиса и дермы при формировании грубого соединительнотканного рубца и эпителизации раны без образования рубца с восстановлением функции поврежденного участка... читать о результатах эксперимента

Watch what you take, otherwise... you'll get better!
Watch what you take, otherwise... you'll get better!

Few of my colleagues remember the glorious time when not only a comprehensive approach based on objective knowledge was used in the treatment of skin diseases and sexually transmitted infections, but also Immunostimulants were used, especially for the treatment of late forms of syphilis. Further...